Label-free, quantitative microscopic imaging, specialized for detection and tracking of biological and non-biological micro-particles, providing high-throughput, volumetric, 3D characterizations of particle motion.
Metro DHM
A turnkey inline digital holographic microscopy (DHM) instrument
Why Metro DHM?
A turnkey inline digital holographic microscopy (DHM) instrument for real world applications.
Label-free
Eliminate influences of fluorescent markers on biologically relevant results
Easy to Operate
Simplified procedures from mounting the sample container to data acquisition
3D
Resolve intrinsically 3D motion, which is otherwise not accessible for conventional microscopy
Compact
Small footprint with a total length less than half a meter
Volumetric
Avoid influences of 2D confinement on biological samples and provide sustained observation of samples through the depth
Affordable
A microscopic imaging instrument capable of 4D characterization does not have to be expensive.
High-throughput
Ideal tool for determining robust statistics
Customizable
Tell us the specialty of your application, we come up with a MetroDHM solution for you!
Metro DHM WORKFLOW
Label-free, quantitative microscopic imaging, specialized for detection and tracking of biological and non-biological micro-particles, providing high-throughput, volumetric, 3D characterizations of particle motion.
imaging preparation
Accommodates sample containers in which particles of interest can move freely in 3D (e.g., 2-mm cuvette). 3-axis micron-precision translation stage for precise sample positioning. No need to mount on an optical table - robust to vibrations
Hologram Acquisition
Observes samples in real time. Convenient and fast in taking large datasets
Data Processing
HoloPro software – our hologram processing and data analysis powerhouse. Ease-to-use realized by intuitive GUI. Exceptional speed realized by GPU-acceleration, ideal for extracting statistics from large dataset
Data Analysis
3D data visualization for particle distribution and trajectories. Calculation of multiple kinematic parameters. Exportation of results in formats readable to other software for additional analysis and visualization
DEMONSTRATION
Application of MetroDHM to bacterial motility research
Our MetroDHM instrument has been used to acquire the motility data of Azospirillum brasilense (A. brasilense), a model soil bacterial specie whose chemotaxis plays an important role in metabolic processes in the rhizosphere. While being imaged, the bacteria are freely swimming in a 2-mm depth cuvette filled with the bacterial minimal medium. Processing of hologram sequences using HoloPro yields 3D trajectories, from which a range of motility parameters as well as motility patterns are extracted. The quantitative results reveal the differences in motility pattern of the wildtype (Sp7) and mutant (cheA4) strains.
Sample: preprocessed hologram sequence acquired at 12 FPS showing freely swimming bacteria
Sample: Sustained, quantitative, 3D observation of a swimming bacterium for ~80 seconds at 12 FPS
Sample: 1885 trajectories, each longer than 5 seconds, determined from a 90-second hologram sequence acquired at 12 FPS
contact us to learn more
MetroLaser will continue to pursue state-of-the-art research and development as well as the commercialization of optical diagnostics systems to measure flow velocity, temperature, chemical composition, surface temperature inside gas turbine engines, and non destructive inspection of composites and other components.