An all-in-one solution for holographic reconstruction
A Robust Tool for Digital Reconstructions, Simulations & Particle Field Holography
Our MetroDHM instrument has been used to acquire the motility data of Azospirillum brasilense (A. brasilense), a model soil bacterial specie whose chemotaxis plays an important role in metabolic processes in the rhizosphere. While being imaged, the bacteria are freely swimming in a 2-mm depth cuvette filled with the bacterial minimal medium. Processing of hologram sequences using HoloPro yields 3D trajectories, from which a range of motility parameters as well as motility patterns are extracted. The quantitative results reveal the differences in motility pattern of the wildtype (Sp7) and mutant (cheA4) strains.
| Hologram formats accepted | tiff, tif, png, bmp, jpeg , jpg |
| Hologram types | In-line & Off-axis (planar waves) |
| Inputs | Object & reference holograms (latter is optional), illumination wavelength, pixel size (length & width), system magnification, cross term location and size (off-axis holography), propagation distance |
| Background removal | Subtraction, Division, SDPM |
| Propagation methods | Angular Spectrum, Upsampling, Lowpass filtering, Fast Fourier Transform |
| Pre-propagation features | Sinc compensation, Zero padding |
| De-twinning | Two methods: Iterative or SDPM |
| Optical distortion removal | Fixed frequency distortion target (dot-pattern grid) calibration |
| Simulations | Simulate field-of-view, fringes captured, lateral resolution based on imaging sensor, setup and illumination of a spherical object |
| Particle field holography | Determine 3D location and size of particles in sequential holograms using minimum intensity and maximum Tenengrad methods |
| Measurements | Amplitude and Phase (wrapped/unwrapped), 3D particle trajectories, mean velocities, instantaneous velocities |
| Reconstruction(s) | Single or batch processing |
| Save/Load | Settings, reconstruction, simulation |
| Operating system | Windows 10 (64 bit) |
| CPU | Intel Core i3 or faster |
| GPU | NVIDIA graphics card with a compute capability of 3.5 and above |
| Memory | 2 GB or more |
| Screen size | 1328 × 510 or larger |
| Connection Port | USB 3.0 |
| License | USB dongle (2.27” × 0.75” × 0.42”) |
MetroLaser will continue to pursue state-of-the-art research and development as well as the commercialization of optical diagnostics systems to measure flow velocity, temperature, chemical composition, surface temperature inside gas turbine engines, and non destructive inspection of composites and other components.
