A turnkey inline digital holographic microscopy (DHM) instrument for label-free, quantitative microscopic imaging, specialized for detection and tracking of biological and non-biological micro-particles, providing high-throughput, volumetric, 3D characterizations of particle motion
Our MetroDHM instrument has been used to acquire the motility data of Azospirillum brasilense (A. brasilense), a model soil bacterial specie whose chemotaxis plays an important role in metabolic processes in the rhizosphere. While being imaged, the bacteria are freely swimming in a 2-mm depth cuvette filled with the bacterial minimal medium. Processing of hologram sequences using HoloPro yields 3D trajectories, from which a range of motility parameters as well as motility patterns are extracted. The quantitative results reveal the differences in motility pattern of the wildtype (Sp7) and mutant (cheA4) strains.